Sheta M Sheta
Inorganic Chemistry Department, National Research Centre, Egypt
Title: Ultrasensitive optical biosensor for cardiac troponin I biomarker based on Mn-MOF
Biography
Biography: Sheta M Sheta
Abstract
For the first time, a simple, fast technique, costless, room temperature, high accuracy, sensitivity and selectivity optical biosensor for cardiac troponin I (cTn) as an early test for myocardial infarction diagnosis could be addressed. It is also facilitated and helped an emergency departments (EDs) decision-making in patients with chest pain. A manganese metal-organic framework (Mn-MOF) synthesized via a simple method, and then fully characterized. A photoluminescence (PL) study based on Mn-MOF aganist cTn was investgated. The results showed that the Mn-MOF exhibited a strong emission at 422 nm with excitation at 300 nm at room temperature. Upon addtion of cTn a redshift with remarkable quenching carried out and new emission band appaeared at 397 nm. By applying the Stern-Volmer graph a linear correlation achieved over a concentration range 0.01-25 pg/mL of cTn with a correlation coefficient, 0.989, limit of detection and quantitation 0.01 pg/mL and 0.03 pg/mL, respectively. According to the significant quenching and redshift of the Mn-MOF PL intensities upon using different cTn concentrations was revealed. The Mn-MOF may be used successfully as optical biosensor for cTn in different biological samples (serum, plasma and whole blood). Moreover, the Mn-MOF showed a high selectivity towards cTn competing with other different interfering analytes. The quenching mechanism well studied and it has a dynamic type and obtained due to the energy transfer mechanism.